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KMID : 1007520030120010079
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Food Science and Biotechnology
2003 Volume.12 No. 1 p.79 ~ p.82
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Isolation and Purification of Anticancer Peptides from Korean Ginseng
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Kim, Sun-ho
Kim, Ji Yeon/Park, Sung Won/Lee, Ki Won/Kim, Kyoung Heon/Lee, Hyong Joo
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Abstract
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To investigate anticancer activity of ginseng, peptides were isolated from Panax ginseng C.A. Meyer, and analyzed for their anticancer activity. Water extract of ginseng powder was fractionated by hydrophobic XAD-2 column chromatography using 100% ethanol as an eluant. The hydrophobic fraction was found to show high anticancer activity on cell line (P388D1, a mouse lymphoma) with and IC_50 of 13.3 μg/mL. According to amino acid analysis and sequencing result, the anticancer peptides were composed of tryptophan with a sequence of either trp-trp or trp-trp-trp. The synthetic tripeptide, Trp-Trp-Trp, had the highest anticancer activity on P388D1 cell, whereas Trp in free amino acid form exhibited no effects on the cell. IC_500 values of dipeptide and tripeptide were 442.9 and 184.9 μg/mL, respectively. In the DNA cell cycle analysis of the tumor cell treated with the peptide fraction by flow cytometry, the DNA contents of S and G2/M phases in the cell cycle decreased, whereas that of G0/G1 phases increased.
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KEYWORD
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